L3-GEN-TISS-FIBR
ESSENTIAL
PRESERVATION
Level 3 · hardware
Fibroblast Cell Line Archive
Cryopreserved Fibroblast Cell Culture Collection
Archive of cryopreserved fibroblast cell lines representing maximum species diversity
Purpose
Archive of cryopreserved fibroblast cell lines representing maximum species diversity
Context
Child of L2-GEN-TISS
Principles
- ▸Fibroblasts are most commonly banked cell type - easy to establish from skin biopsies
- ▸Controlled-rate freezing in DMSO/FBS (fetal bovine serum) is standard preservation method
- ▸Multiple vials per cell line (5-10) provide redundancy against individual vial failure
- ▸Cell passage number tracked to ensure low-passage cultures for maximum genetic integrity
Typical implementations
- ▸San Diego Zoo Wildlife Alliance Frozen Zoo (10,000+ cell cultures, 1,100+ species)
- ▸ATCC (American Type Culture Collection) cell banking standards
- ▸Coriell Institute for Medical Research biobank operations
Lunar considerations
- ▸Cell viability after centuries of cryopreservation is unknown beyond ~50 years data
- ▸Cells contain complete nuclear and mitochondrial DNA for potential cloning
- ▸Lower priority than DNA/seeds for species where those alternatives exist
Specifications
Functional
| primary function | Archive of cryopreserved fibroblast cell lines representing maximum species diversity |
Physical
| materials | Cryovials (2mL), DMSO cryoprotectant, FBS preservation medium |
Interfaces
Provides
- Cryovials stored in cryogenic dewars
Requires
- Species priority list for cell banking
Cite this entry
Lunar Ark Codex. "Fibroblast Cell Line Archive" (L3-GEN-TISS-FIBR). Retrieved 10 September 2026, from https://lunarark.com/entry/L3-GEN-TISS-FIBR
Licensed CC-BY-SA 4.0. You may reuse and adapt this entry with attribution, under the same licence.